The aim of this study was to evaluate salivary characteristics, including salivary fluoride (F), calcium (Ca), phosphorus (P), protein, amylase, lysozyme, and lactoferrin levels, in 60 children (selected from 420 examined) who were divided into three groups: (1) children with dental fluorosis, (2) children without dental fluorosis and healthy teeth, but no caries, and both groups living in Isparta, Turkey with high F (2.7-2.8 ppm) water, and (3) children without dental fluorosis and healthy teeth living in the nearby city of Afyon with low F (0.153 ppm) water. No statistical differences between groups were found except for lysozyme levels. Further studies on the effect of different water F levels on salivary composition are considered desirable.