<p>Commercial bumblebee colonies have been used for the pollination of a number of crops, mainly of tomatoes (95%). In<br />comparison with other bumblebee species, Bombus terrestris has the most year round rearing. The year round rearing<br />procedure of B. terrestris involves some main stages: colony foundation, obtaining of young queens and males, mating<br />and diapause control. Success of these stage are affected by environmental conditions, physiological properties and<br />genetic structure ofthe queens. Knowledge of the effects of these factors will increase the rearing success. Because of<br />the B. terrestris queens mate once, female individuals in their colonies are supersister. There is a 75% genetic<br />relatedness between the supersister young queens. This study was conducted to compare the different super sister queen<br />groups and to determine the effect of genotype on weight, mating performance and diapause performance of queensin<br />B. terestris. Results showed that genetic differences affected the copulation duration and pre and post diapause weight<br />of queens, but survival ratio during the diapause duration did not affected by genotypic differences.</p>