This study was carried out to detect of Tobacco mosaic virus (TMV) in tobacco growing areas in Denizli province, Turkey. Total of 94 plant samples including mosaic, curling of leaves, chlorotic lesion and stunding symptoms were collected from the fields in Denizli province during 2015-2016 years. Presence of Tobacco mosaic virus was investigated using Double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) method. As a result of DAS-ELISA, it was found 30.85% of leaf samples with TMV.