Effective hemorrhage control with materials developed by emerging technologies is important in preventing massive bleeding in hospitals, accident sites and battlefields. Various materials have been developed for the treatment of hemorrhage. Gelatin is one, which is commonly used in combination with thrombin. It is generally used in internal bleeding cases owing to its safety, and superior biocompatibility and biodegradability. On the other hand, thrombin the main component that provides rapid haemostasis has drawbacks such as serious disease transmission from donors and high cost. Chitosan is another haemostatic material component with low cost and superior efficiency. However, chitosan-based haemostatic materials are not recommended for internal use. In this paper, we examine the use of a combination of gelatin and tranexamic acid microparticles in internal bleeding control, on the basis of comparative studies both in in vitro and in in vivo conditions. This composite haemostatic material provided rapid haemostasis and biocompatibility, and provided a safe use in internal applications.