Arabinogalactan-proteins (AGPs) are classified in the group of proteoglycans and useful substrates for different part of plants. There are kind of effects of AGPs on the events of plant growing and improvement which are supported by embryogenesis and cell division. In this study, effect of AGP has been investigated on plant regeneration in wheat plants using calli derived from mature embryos. Calli developed were produced by cultivating mature embryo with some selected hormones and their combinations (auxin and cytokinin) and differences found in increasing of either calli number or weight statistically. Murashige and Skoog (MS) medium was used for AGP application in plant regeneration stage of this tissue culture study. AGP increased regeneration capacity and culture effects of genotypes significantly (P < 0.05). It can be said that AGP (10 mg/l) was found to be useful substance for wheat tissue culture to increase plant regeneration. However 10 mg/l of AGP did not make difference statistically between the capacity of plant regeneration in tetraploid and hexaploid genotypes.