In this study, we aimed to determine the genetic diversity of wheat genotypes grown in Isparta and Burdur provinces, to protect valuable genotypes as a germplasm source and to take them into breeding programs. In the study, about 104 villages were visited and 72 wheat seed samples were taken from 45 different locations. The resistance of the sampled wheat varieties/populations to yellow rust disease was examined and the following results were obtained; the rate of resistant genotypes (R) was 6% (disease severity 1-2%), the rate of moderate resistant genotypes (MR) was 33% (disease severity 6-20%), the LM rate was 33% (21-40%), the rate of those in the M group was 19% (disease severity 41-60%), and the rate of HM, which has a little less tolerance, was 8% (disease severity % 61-80). Very few of the collected material was found to be resistant (6%) and most of the material was tolerant (85%).