The objective of this study was to determine the accuracy of body measurements (BM) in Holstein female calves using digital image analysis. BM including body length, wither height, chest depth, hip height, and hip width of calves were recorded by stick and tape measurements at birth, weaning and 24 weeks of age. Then photos of calves were taken while calves were standing in a squeeze chute by a digital camera and were analysed by image analysis software to obtain BM of each calf from the image in centimetres. After comparing the actual and predicted BM, the accuracy was determined as 71, 97 and 99% for body length, 69, 87 and 99% for wither height, 43, 98 and 99% for chest depth, 74, 99 and 99% for hip height and 53, 99 and 98% for hip width at birth, weaning and 24 weeks of age, respectively. The difference between actual and predicted BM was significant at birth (P < 0.01). Although there were numerical differences between actual and predicted BM, the differences were not significant at weaning and 24 weeks of age (P > 0.01). According to these results, the BM estimation of Holstein female calves using digital image analysis produced high prediction accuracy at weaning and 24 weeks of age, but not at birth. The data presented in this study indicate that the digital image analysis provides very close agreement and reality for prediction of BM of Holstein female calves.