The purpose of the study is to investigate the damage caused by iron (Fe) and zinc (Zn) heavy metals in the testis tissue of rats and the protective impact of juglone an antioxidant in preventing this damage- with the help of histological and immunohistochemical methods. Adult, male Wistar-Albino rats raised in Suleyman Demirel University Experimental Animals Production and Experimental Research Laboratory (HADYEK) were used in this study. Subjects were randomly grouped into 5. Group I; iron (Fe) (iron from 0.3 ml stock solution/600 ppm. + 0.7 ml.water), Group II; zinc (Zn) (zinc from 0.2 ml stock solution/400 ppm + 0.8 ml.water), Group III; iron (iron from 0.3 ml stock solution /600 ppm.) + Antioxidant (juglone (5-hidroxy-1,4-naphthoquinone)/200 ppm- 0.7 ml.), Group IV; zinc (zinc from 0.2 ml stock solution/400 ppm.)+Antioxidant (juglone (5-hidroxy-1,4naphthoquinone)/200 ppm-0.7 ml.+ 0.1 ml. water), Group V; Control (1 ml. Water); gavage was applied to each group once a day throughout 30 days. At the end of the experiment, rats were decapitated and their testis tissues removed, then histological staining and TUNEL method were applied following the routine histological follow-ups. Based on the histopathological examinations, significant damage was observed in the testis tissue of the groups administered with Fe and relatively Fe+Juglone. The most explicit findings were; partly ruptures in seminiferous tubules epithelium and decrease in thickness of epithelium, dissociation in basal lamina of the seminiferous tubules, decrease in the number of germ cells, intense edema and congestion in the interstitial area, and vacuolization in the seminiferous tubules epithelium. The damage associated with the Zn and Zn+Juglone was determined to be less significant than the damage by Fe and Fe+Juglone group. Based on the TUNEL method, a significant increase was observed in the number of apoptotic cells in Fe, Fe+Juglone and Zn groups compared to the control group (p<0.05). No significant difference was observed between the Zn, Zn+Juglone group and control group.