In construction sector, the necessity to cement-based materials having superior properties is increasing day by day. In this study, novel forms of new generation polycarboxylate-based superplasticizers which provide higher fluidity to concrete mixtures were synthesized, characterized and their performances in cement pastes containing silica fume were tested. The influences of polymers possessing different molecular structures on the flow properties of cement pastes were investigated with mini-slump and rheology tests. Two different model approaches as Bingham and Herschel-Bulkley were used to examine rheological behaviours. It was observed that, the existence of dicarboxylic acid as fumaric acid in superplasticizer structure have effects on pastes' workability and the time-dependent retention of this workability. It was determined that, synthesized superplasticizers increase the flow diameter of paste over 100% than plain paste and can be preferable for the effective fluidity behaviours of mixtures by time (2 hours). Additionally comparing with mortars containing only cement as binder, it was seen that superplasticizers in question improve the mechanical strengths of mortars possessing cement and silica fume. As a result, it was understood that the synthesized new generation polycarboxylates have potential to be used in high performance concrete production.