In this research, the effects of daminozide (Alar 85) treatment on the stomata movements in the leaves of two spray chrysanthemum cultivars (Yellow Reagan and White Reagan) grown in both short and natural day conditions were studied. In Yellow Reagan, the epidermis cell number, stomata number, stomata index and stomata width in the leaves of control seedlings grown in both short and natural day conditions were more in the lower than the upper surface. In addition, the stomata of control seedlings grown in short day conditions was longer in the lower than in the upper surface. As for the natural day conditions, the stomata of control was statistically in the equal length in both surfaces. In White Reagan, the stomata number, stomata index and stomata width in the leaves of control seedlings grown in both short and natural day conditions were more in the lower than the upper surface. Moreover, the epidermis cell number and stomata length of control seedlings grown in natural day conditions were higher in the upper than in lower surface. As for the short day conditions, the epidermis cell number of control was higher in the lower than upper surface, while the stomata of control was statistically in the equal length in both surfaces. On the other hand, it was observed that daminozide (Alar 85) treatment affected in different degrees on the stomata movements in the leaves of both Chrysanthemum cultivars and this difference was statistically important.