In this study, the relationship between chemical characteristics of chips, UF-Resin ratios and physical, mechanical properties of particleboard manufactured from vine prunings was investigated. Before particleboard manufacturing, the chips produced from vine prunings were subjected to chemical pretreatment such as cold water treatment (CW), 1% NaOH treatment (N) and 1% CH(3)COOH treatment (A). FTIR spectra of chips were demonstrated that, the content of OH groups was increased by pre-treatments. Therefore, organic chemical component contents (extractives, holocellulose and lignin) of chips were determined. After producing particleboards, physical and mechanical properties namely; water absorption (WA), thickness swelling (TS), modulus of elasticity (MOE), modulus of rupture (MOR) and internal bond strength (IB) were studied. The multiple linear regression models were developed using physical and mechanical properties of particleboard as dependent variables and extractive, holocellulose, lignin contents and UF-Resin ratio as independent variables. The correlation coefficient (R(2)) values of 0.90 and 0.92 for estimating TS and WA confirmed that models produced more accurate results for physical properties than mechanical properties (R(2) values of 0.64, 0.85 and 0.89 for estimating MOE, MOR and IB, respectively).