In the study, it was aimed to determine the optimum irrigation issue by examining the anatomical features of Lycopersicon esculentum Mill. cv. Ceren cultivated in perlite by different irrigation treatments. Irrigation strategies were created based on the amount of drainage water and different irrigation applications were performed by using the drip irrigation system. For anatomical examination, cross sections were taken from the samples of the root, stem and leaf by microtome and examined by light microscopy. Some tissues in the root, stem, and leaf under over-irrigation and limited irrigation conditions changed significantly. As a result, the condition created by reducing 25% of irrigation water was determined as the optimum irrigation issue in four different irrigation applications. The amount of drainage under optimum condition was 10% of the amount of irrigation water applied. The study will contribute to the researchers to optimize the ecological condition in terms of irrigation in greenhouse.