Sainfoin has little atention for plant tissue culture studies. However, genetic engineering applications of gene transfer can be done today. Therefore, first different methods are used in tissue culture (For example, using of different hormones for plant growing) and be given importance. In this study, the effect of different concentration (0-5-10-20-30 ppm) sage (Salvia officinalis L.) and Turkish oregano (Origanum onites L.) have profound effects on morphology of the tissues that grow from the initial sainfoin explant. The study was arranged in factorials with 6 replications and of variance was done using statistical analysis system (SAS) software. The data collected included the rate of seed germination, plant length, root number and root length. The highest seed germination, plant length and root length was obtained on sage volatile oil. Used both volatile oil reduced the germination ratio. Sage increased the plant and root length. Even so, Turkish orego significantly reduced plant length and is root number increased.