Objective: With an increasing incidence and prevalence, Chronic Kidney Disease (CKD), is a bad prognosed and high cost common public health problem. KLOTHO gene which is defined as aging suppressor gene is highly expressed in kidneys. Decreased Vitamin D receptor (VDR) activation plays a role in CKD morbidity and mortality. The aim of this study is to examine the association between CKD and polymorphisms of KLOTHO (G395A and C1818T) and VDR (Apal and Taql) genes as well as investigating the effects of these polymorphisms on different clinical phenotype of the disease.