Background: Atopic dermatitis (AD) is a chronically relapsing skin disease that occurs most commonly during early infancy and childhood. The pathogenesis of AD has not been fully elucidated. The interaction of SCF (stem cell factor) and its receptor, tyrosine kinase transmembrane receptor (KIT), appears to be key event in the recruitment and proliferation of mast cells in AD. Materials and Methods: In this study were measured serum levels of sSCF and sKIT using ELISA of 25 patients with AD and 25 healthy individuals as a control group. Results: Serum levels of sSCF and sKIT were significantly higher in AD patients than in controls (p<0,05). Although we detected a positive correlation between serum levels of sSCF and sKIT, this correlation was not statistically significant (p>0,05). Serum levels of sSCF and sKIT were significantly higher in patients with severe AD than in patients with moderate AD (p<0,05). Conclusion: Serum sSCF and sKIT levels may be useful indicators for the evaluation of the disease activity and severity in AD patients.