<p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;line-height:150%"><i><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;mso-fareast-theme-font:minor-fareast;mso-ansi-language:EN-US;mso-fareast-language:TR">Origanum onites</span></i><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;mso-fareast-theme-font:minor-fareast;mso-ansi-language:EN-US;mso-fareast-language:TR"> L. is one of the mainaromatic species exported from Turkey with medicinal properties. It is known tohave antiseptic, antibacterial, antispasmodic, antifungal, </span><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US">antimicrobial, anti-inflammatory</span><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;mso-fareast-theme-font:minor-fareast;mso-ansi-language:EN-US;mso-fareast-language:TR"> and anticarncinogenic propertiesdue to its volatile oils and terpenic materials<span style="color:red">. </span>Plantproduction using the tissue culture technique is a method of production thatenables medicinal and aromatic plants to be reproduced vegetatively rapidly andin large quantities, as in many plant species. </span><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-ansi-language:EN-US">Strigolactones are associated with regulatory roles inplant growth and development, including seed germination, root and shootarchitecture patterning, nutrient acquisition, symbiotic interactions, as wellas mediation of plant responses to abiotic and biotic cues.</span><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;mso-fareast-theme-font:minor-fareast;color:red;mso-ansi-language:EN-US;mso-fareast-language:TR"> </span><span lang="EN-US" style="font-size:12.0pt;line-height:150%;font-family:&quot;Times New Roman&quot;,&quot;serif&quot;;mso-fareast-font-family:&quot;Times New Roman&quot;;mso-fareast-theme-font:minor-fareast;mso-ansi-language:EN-US;mso-fareast-language:TR">In this work, we aimed toevaluate the effect of a new class phytohormone strigolactone on <i>in vitro</i>callus formation of Oregano plant. Callus cultures were obtained by initiatingleaf explant on Murashige and Skoog (MS) medium supplemented with6-Benzylaminopurine (BAP; 2.0 mg l^-1) in combination with 1-<span style="color: rgb(34, 34, 34); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">Naphthalene acetic acid (NAA; 1.0 mg l^-1) andvarious concentrations of strigolactones (0.0, <a name="_GoBack"></a>0.1, 0.5,1.0, 2.0 and 5.0 µM) were added. I</span>n each stage of the experiment,explants placed on MS media with 2.0 mg l^-1BAP and 1.0 mg l^-1NAAwithout the addition of strigolactone were the control. After 21 days of callusinduction, it was found that all the explants produced a compact callus.Furthermore, after 35-40 days during the callus multiplication stage, it wasobserved that the best mass of the callus tissue was the media supplementedwith 2.0 <span style="color: rgb(34, 34, 34); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">µM strigolactone. It wasalso observed that the mass of the propagated callus tissue decreased with anincrease of strigolactone. The results obtained indicate that a lowerconcentration of strigolactone has positive impact on callus development. Thisprotocol of ours presents the first of its kind and will lead the way forfuture studies.<o:p></o:p></span></span></p>