Sex-workers are considered as the high-risk population for sexually transmitted infections (STIs). Early diagnosis and treatment of curable STIs in this high-risk group have crucial importance in STI control and prevention of complications and transmission of infection. In this study, 146 registered female sex-workers in Ankara city were screened with rapid diagnostic tests (RDT) for causative agents of curable STIs such as, Neisserio gonorrhoeoe, Chlomydia trachomatis, Trichomonas voginalis and Treponerna pollidum. To identify gonorrhoea, Gram stained enclocervical smears were examined microscopically for the presence of gram-negative intracellular cliplococci. For the diagnosis of chlamydial infection, an optic immunoassay (OIA) (Chlomydic OIA, Biostar, USA) as a RDT was performed by using enclocervical specimens. For the detection of Tvaginolis, direct smears of vaginal swabs were examined for the presence of motile trophozoites first directly and after being cultured in Diamond's media for 24-48 hours of incubation. Syphilis was screened in the serum specimens by RPR (Omega, UK) test. There was no positive test results for gonorrhoea and syphilis however, the frequency of Ctrachomatis and Tvaginolis in the study population was 1.4% and 0.7%, respectively. To provide comprehensive policies and optimal control strategies, a reliable source of data about the frequency and spectrum of STIs among high-risk populations and optimized effective screening programmes are required.