Description:
<p><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;">This study aimed to determine the chromatographic retention and dissociation/protonation constant (pK</span><span style="margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif;">a</span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;">) values of lapatinib and tamoxifen, key drugs used in metastatic breast cancer treatment, at 37°C using both conventional and green high-performance liquid chromatography (HPLC) methods. Qualitative analysis was conducted on an XTerra C18 column (250 ×4.6 mm I.D., 5 μm particle size) at a flow rate of 1 mL/min. Hydroorganic mixtures with 45 %, 50 %, 55 %, and 60 % (v/v) organic modifiers were used to evaluate the retention times of the compounds. The compatibility of pK</span><span style="margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif;">a</span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"> values </span><span class="math" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><span class="MathJax_Preview" style="margin: 0px; padding: 0px; color: rgb(136, 136, 136);"></span><span class="MathJax_SVG" id="MathJax-Element-1-Frame" tabindex="0" data-mathml="<math xmlns="http://www.w3.org/1998/Math/MathML"><mrow is="true"><mfenced close=")" open="(" is="true"><mrow is="true"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">a</mi></mrow><none is="true" /><mprescripts is="true" /><mrow is="true"><mi is="true">s</mi></mrow><mrow is="true"><mi is="true">s</mi></mrow></mmultiscripts></mrow></mfenced></mrow></math>" role="presentation" style="margin: 0px; padding: 0px; display: inline-block; line-height: normal; font-size: 14.4px; font-size-adjust: none; word-spacing: normal; overflow-wrap: normal; text-wrap-mode: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; position: relative;"><svg xmlns:xlink="http://www.w3.org/1999/xlink" width="7.115ex" height="2.779ex" viewBox="0 -846.5 3063.4 1196.3" role="img" focusable="false" style="vertical-align: -0.812ex;" aria-hidden="true"><g stroke="currentColor" fill="currentColor" stroke-width="0" transform="matrix(1 0 0 -1 0 0)"><g is="true"><g is="true"><use xlink:href="#MJMAIN-28"></use><g is="true" transform="translate(389,0)"><g is="true"><g is="true" transform="translate(406,0)"><g is="true"><use xlink:href="#MJMATHI-70"></use><use xlink:href="#MJMATHI-4B" x="503" y="0"></use></g></g><g transform="translate(0,410)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-73"></use></g></g></g><g transform="translate(1834,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-61"></use></g></g></g><g transform="translate(0,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-73"></use></g></g></g></g></g><use xlink:href="#MJMAIN-29" x="2673" y="0"></use></g></g></g></svg><span class="MJX_Assistive_MathML" role="presentation" style="margin: 0px; top: 0px; left: 0px; clip: rect(1px, 1px, 1px, 1px); user-select: none; transition: none; padding: 1px 0px 0px !important; position: absolute !important; border: 0px !important; height: 1px !important; width: 1px !important; overflow: hidden !important; display: block !important;"><math xmlns="http://www.w3.org/1998/Math/MathML"><mrow is="true"><mfenced close=")" open="(" is="true"><mrow is="true"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">𝑎</mi></mrow><none is="true"></none><mprescripts is="true"></mprescripts><mrow is="true"><mi is="true">𝑠</mi></mrow><mrow is="true"><mi is="true">𝑠</mi></mrow></mmultiscripts></mrow></mfenced></mrow></math></span></span></span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"> of the compounds in water-organic solvent mixtures obtained from these studies, which were carried out without any significant change in liquid chromatography performance, with the values obtained by the conventional method is remarkable. The </span><span class="math" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><span class="MathJax_Preview" style="margin: 0px; padding: 0px; color: rgb(136, 136, 136);"></span><span class="MathJax_SVG" id="MathJax-Element-2-Frame" tabindex="0" data-mathml="<math xmlns="http://www.w3.org/1998/Math/MathML"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">a</mi></mrow><none is="true" /><mprescripts is="true" /><mrow is="true"><mi is="true">s</mi></mrow><mrow is="true"><mi is="true">s</mi></mrow></mmultiscripts></math>" role="presentation" style="margin: 0px; padding: 0px; display: inline-block; line-height: normal; font-size: 14.4px; font-size-adjust: none; word-spacing: normal; overflow-wrap: normal; text-wrap-mode: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; position: relative;"><svg xmlns:xlink="http://www.w3.org/1999/xlink" width="5.306ex" height="2.663ex" viewBox="0 -796.9 2284.4 1146.6" role="img" focusable="false" style="vertical-align: -0.812ex;" aria-hidden="true"><g stroke="currentColor" fill="currentColor" stroke-width="0" transform="matrix(1 0 0 -1 0 0)"><g is="true"><g is="true" transform="translate(406,0)"><g is="true"><use xlink:href="#MJMATHI-70"></use><use xlink:href="#MJMATHI-4B" x="503" y="0"></use></g></g><g transform="translate(0,410)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-73"></use></g></g></g><g transform="translate(1834,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-61"></use></g></g></g><g transform="translate(0,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-73"></use></g></g></g></g></g></svg><span class="MJX_Assistive_MathML" role="presentation" style="margin: 0px; top: 0px; left: 0px; clip: rect(1px, 1px, 1px, 1px); user-select: none; transition: none; padding: 1px 0px 0px !important; position: absolute !important; border: 0px !important; height: 1px !important; width: 1px !important; overflow: hidden !important; display: block !important;"><math xmlns="http://www.w3.org/1998/Math/MathML"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">𝑎</mi></mrow><none is="true"></none><mprescripts is="true"></mprescripts><mrow is="true"><mi is="true">𝑠</mi></mrow><mrow is="true"><mi is="true">𝑠</mi></mrow></mmultiscripts></math></span></span></span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"> values determined in this study were correlated with the macroscopic parameters of acetonitrile, methanol, ethanol and the pK</span><span style="margin: 0px; padding: 0px; font-size: 12px; line-height: 0; position: relative; vertical-align: baseline; bottom: -0.25em; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif;">a</span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"> (</span><span class="math" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><span class="MathJax_Preview" style="margin: 0px; padding: 0px; color: rgb(136, 136, 136);"></span><span class="MathJax_SVG" id="MathJax-Element-3-Frame" tabindex="0" data-mathml="<math xmlns="http://www.w3.org/1998/Math/MathML"><mrow is="true"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">a</mi></mrow><none is="true" /><mprescripts is="true" /><mrow is="true"><mi is="true">w</mi></mrow><mrow is="true"><mi is="true">w</mi></mrow></mmultiscripts><mrow is="true"><mo stretchy="false" is="true">)</mo><mspace width="0.25em" is="true" /></mrow></mrow></math>" role="presentation" style="margin: 0px; padding: 0px; display: inline-block; line-height: normal; font-size: 14.4px; font-size-adjust: none; word-spacing: normal; overflow-wrap: normal; text-wrap-mode: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; position: relative;"><svg xmlns:xlink="http://www.w3.org/1999/xlink" width="7.197ex" height="2.779ex" viewBox="0 -846.5 3098.6 1196.3" role="img" focusable="false" style="vertical-align: -0.812ex;" aria-hidden="true"><g stroke="currentColor" fill="currentColor" stroke-width="0" transform="matrix(1 0 0 -1 0 0)"><g is="true"><g is="true"><g is="true" transform="translate(581,0)"><g is="true"><use xlink:href="#MJMATHI-70"></use><use xlink:href="#MJMATHI-4B" x="503" y="0"></use></g></g><g transform="translate(0,410)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-77"></use></g></g></g><g transform="translate(2009,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-61"></use></g></g></g><g transform="translate(0,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-77"></use></g></g></g></g><g is="true" transform="translate(2459,0)"><g is="true"><use xlink:href="#MJMAIN-29"></use></g><g is="true"></g></g></g></g></svg><span class="MJX_Assistive_MathML" role="presentation" style="margin: 0px; top: 0px; left: 0px; clip: rect(1px, 1px, 1px, 1px); user-select: none; transition: none; padding: 1px 0px 0px !important; position: absolute !important; border: 0px !important; height: 1px !important; width: 1px !important; overflow: hidden !important; display: block !important;"><math xmlns="http://www.w3.org/1998/Math/MathML"><mrow is="true"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">𝑎</mi></mrow><none is="true"></none><mprescripts is="true"></mprescripts><mrow is="true"><mi is="true">𝑤</mi></mrow><mrow is="true"><mi is="true">𝑤</mi></mrow></mmultiscripts><mrow is="true"><mo stretchy="false" is="true">)</mo><mspace width="0.25em" is="true"></mspace></mrow></mrow></math></span></span></span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;">values of lapatinib and tamoxifen in water were calculated. The </span><span class="math" style="margin: 0px; padding: 0px; color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"><span class="MathJax_Preview" style="margin: 0px; padding: 0px; color: rgb(136, 136, 136);"></span><span class="MathJax_SVG" id="MathJax-Element-4-Frame" tabindex="0" data-mathml="<math xmlns="http://www.w3.org/1998/Math/MathML"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">a</mi></mrow><none is="true" /><mprescripts is="true" /><mrow is="true"><mi is="true">w</mi></mrow><mrow is="true"><mi is="true">w</mi></mrow></mmultiscripts></math>" role="presentation" style="margin: 0px; padding: 0px; display: inline-block; line-height: normal; font-size: 14.4px; font-size-adjust: none; word-spacing: normal; overflow-wrap: normal; text-wrap-mode: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; position: relative;"><svg xmlns:xlink="http://www.w3.org/1999/xlink" width="5.711ex" height="2.663ex" viewBox="0 -796.9 2459.1 1146.6" role="img" focusable="false" style="vertical-align: -0.812ex;" aria-hidden="true"><g stroke="currentColor" fill="currentColor" stroke-width="0" transform="matrix(1 0 0 -1 0 0)"><g is="true"><g is="true" transform="translate(581,0)"><g is="true"><use xlink:href="#MJMATHI-70"></use><use xlink:href="#MJMATHI-4B" x="503" y="0"></use></g></g><g transform="translate(0,410)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-77"></use></g></g></g><g transform="translate(2009,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-61"></use></g></g></g><g transform="translate(0,-248)"><g is="true"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMATHI-77"></use></g></g></g></g></g></svg><span class="MJX_Assistive_MathML" role="presentation" style="margin: 0px; top: 0px; left: 0px; clip: rect(1px, 1px, 1px, 1px); user-select: none; transition: none; padding: 1px 0px 0px !important; position: absolute !important; border: 0px !important; height: 1px !important; width: 1px !important; overflow: hidden !important; display: block !important;"><math xmlns="http://www.w3.org/1998/Math/MathML"><mmultiscripts is="true"><mrow is="true"><mi mathvariant="italic" is="true">pK</mi></mrow><mrow is="true"><mi is="true">𝑎</mi></mrow><none is="true"></none><mprescripts is="true"></mprescripts><mrow is="true"><mi is="true">𝑤</mi></mrow><mrow is="true"><mi is="true">𝑤</mi></mrow></mmultiscripts></math></span></span></span><span style="color: rgb(31, 31, 31); font-family: ElsevierGulliver, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif, sans-serif; font-size: 16px;"> values calculated from these studies are compatible with each other and with the literature values. The environmental impact of the study, which was carried out using the green method and the conventional RPLC method, was evaluated using the Green Solvent Selection Tool (GSST), Green Analytical Procedures Index (GAPI), and Analytical Greenness Metric Approach (AGREE).</span></p>