Aim: The present study aimed to investigate the effect of the derotation method of Kinesio Taping in addition to conventional rehabilitation in children with hemiplegic cerebral palsy with femoral internal rotation deformity. Materials and Methods: A total of 30 children with unilateral hemiplegic spastic cerebral palsy were randomized into the Kinesio Taping and control group (Conventional Rehabilitation). All included participants have spasticity-related rotation deformity and in-toeing gait. Kinesio Taping group was treated with derotation-tape in addition to conventional rehabilitation. Data was collected before and immediately after the intervention, and also 3 days after the first assessment. The 6-Meter Walk Test, Pediatric Berg Balance Test, Modified Ashworth Scale, Edinburgh Visual Gait Score and Generic Quality-of-Life Instrument for Children were used to assess the children. Results: There was no significant difference between the groups regarding spasticity scores (p>0.05). Spasticity was decreased in hip internal rotators and knee flexors in immediate and acute periods after the intervention in the Kinesio Taping group (p<0.05). There was no difference between the two groups regarding balance, walking, and quality-of-life (p>0.05). Post-intervention improvement in 6- Meter Walk Test, Pediatric Berg Balance Test and Edinburgh Visual Gait Score scores was significant only in the Kinesio Taping group (p<0.05). Quality-of-life increased significantly after treatment in both groups. Conclusion: The results revealed that derotation Kinesio Taping reduces spasticity in the hip internal rotator and knee flexor muscles in the immediate and acute period in children with spastic hemiplegic cerebral palsy. Besides, Kinesio Taping improved balance and walking in the acute period.