BACKGROUND: This study aims to examine the effect of Leukocyte-Rich Platelet-Rich Plasma (LR-PRP) on bacterial translocation in an experimental peritonitis model in rats. Secondary peritonitis occurs due to the loss of integrity in the mucosal barrier of the gastrointestinal system, resulting from contamination of the peritoneal cavity by microorganisms. LR-PRP has been shown to have positive anti-infectious, immunomodulatory, and angiogenetic effects. METHODS: Twenty-seven Wistar-Albino rats were divided into three groups: Sham, Control, and Experimental. Laparotomy was performed on the rats under anesthesia, and the cecum was isolated. No procedure was performed on the Sham group. The cecums of the rats in the Control and Experimental groups were punctured twice within 5 minutes using an 18-gauge needle. The blood product of each rat in the Experimental group was prepared for autologous use as LR-PRP and administered intraperitoneally. The abdomens of rats in all groups were closed after 8 minutes. After 8 hours, the rats were sacrificed, and tissue and blood samples were collected. Inflammatory parameters (TNF-α, IL-1, and IL-6) and blood cultures were analyzed from the blood samples. Cultures were also performed on liver, spleen, and mesenteric lymph node tissue samples. RESULTS: Liver tissue culture growth was not detected in rats in the sham group. It was detected in 6 rats in the control group, and in 1 rat in the experimental group. Mesenteric lymph node tissue culture growth was detected in 2 rats in the sham group, in 7 in the control group and in 1 in the experimental group. Blood culture growth was not detected in rats in the sham group, but detected in 8 rats in the control group, and 3 in the experimental group. In terms of liver tissue culture, mesenteric lymph node tissue culture, and blood culture; a significant relationship was statistically observed between the control and experimental groups (p=0.049, p=0.008, p=0.015, respectively). It was infered that a statistically significant relationship in the mean TNF-alpha, IL-1, IL-6 values was not seen between the control and experimental groups (p=0.999, p=0.999, p=0.590, respectively). CONCLUSION: LR-PRP’s ability to suppress bacterial translocation was statistically significant in liver tissue culture, mesenteric lymph node tissue culture, and blood culture when comparing the Control and Experimental groups. LR-PRP was found to be effec-tive in preventing bacterial translocation without suppressing inflammation and exhibited antimicrobial properties as supported by the literature.