There is a total of six insect species belonging to four families from the order Hemiptera, used in the production of red dye as a natural dyestuff. Carmine is obtained from Dactylopius coccus (Costa, 1829) (Hemiptera: Dactylopiidae) feeding on prickly pears (Opuntia spp.). The genus Dactylopius includes a total of 11 species, and none of them except D. coccus were declared as a dye insect. The reported use of Dactylopius opuntiae (Cockerell, 1896) as a natural dye insect, was more widespread than D. coccus, and advantages versus D. coccus were announced for the first time in this study. To prove the hypothesis that D. opuntiae could be used as a natural dyestuff, silk fabric samples were dyed with D. opuntiae, naturally distributed in Cyprus. The light, wet and dry friction fastness values of the dyed fabric samples were tested. Also the carminic acid values of D. opuntiae were analyzed by high performance liquid chromatography analysis and surface imaging analysis (scanning electron microscope equipped with energy dispersive X-ray spectrometer). The results of the fastness values of the dyed fabric samples and the high carminic acid content of D. opuntiae showed that this species has a very high potential to be used as the 7th natural dye insect in the world.