Objectives: It was aimed to investigate the usability of MMP-2 and TIMP-1 levels as biomarkers in the oosteoarticular complications of brucellosis. Methods: The subjects were categorized into three groups as the healthy control group, brucella group, and brucella patients with osteoarticular involvement groups. Before medical treatment, serum samples from patients and control groups were stored at -80ºC until the day of study. MMP-2 and TIMP-1 serum levels were quantified by the ELISA method.Results: Serum level of MMP-2 (mean ± SD) in healthy control group was 1.71 +/- 0.10 ng / mL. Brucella patient group and Osteoarticular complıcation group were 14.3 +/- 2.52 ng / ml 20.65 +/- 2.33 ng / ml respectively (p=0.001). The mean TIMP-1 level in the control group was 3578.96 +/- 67.2 ng / mL, while in the Brucella group, this rate was 998.27 +/- 66.7 ng / mL and in the bone involvement group, 1656. 17 +/- 17.3 ng / ml. The difference between the control group and the brucella patients and the complicated group was statistically significant (p= 0.001). Conclusions: We think that the significant change in serum levels of MMP-2 and TIMP-1 when evaluated together with the radiological method, can be used as a biochemical indicator of the development of osteoarticular complications.