<p>Violacein is a natural violet pigment produced by various bacterial species, especially by the Gram-negative bacteriaChromobacterium violaceum. Violacein has antibacterial, antifungal, antioxidant and anticancer activities and hasvarious applications in food, cosmetic, textile and pharmaceutical industries. In this study, violacein was extractedfrom the Chromobacterium violaceum culture through ultrasonic-assisted (UA) and microwave-assisted (MA)extraction methods using methanol, ethanol, acetone, and ethyl acetate as solvent. Violacein was quantified usingHigh Performance Liquid Chromatography (HPLC). Then, antibacterial and anticancer activities of these extracts wereinvestigated. In this study, for the first time, the violacein extract was obtained by using UA and MA extractionmethods. The highest violacein concentration could be achieved by UAE method with methanol. It was found out thatviolacein extracts had an antibacterial effect against Staphylococcus aureus, S. aureus (MRSA) and Bacillus cereus.The violacein extract had a strong anticancer effect on cervical cancer (HeLa) cells. Finally, the UA and MA extractionmethods were successfully applied to bacterial cultures in order to extract the violacein.<br></p>